Simultaneous Determination of Doxylamine Succinate, Pyridoxine Hydrochloride and Folic Acid by Chemometric Spectrophotometry

Simultaneous estimation of three component tablets containing Doxylamine succinate (DOX), Pyridoxine hydrochloride (PYR) and Folic acid (FA) was carried out by UV spectrophotometric assisted chemometric methods. Four chemometric methods i.e. classical least square (CLS), inverse least square (ILS), principal component regression (PCR) and partial least squares (PLS) were applied to simultaneous assay of DOX, PYR and FA in tablets without any chemical separation and any graphical treatment of the overlapping spectra of three drugs. The chemometric calculations were performed by using the Chemometrics Toolbox 3.02 software (Kramer, 1995) along with MATLAB 6. The results of four chemometric methods were statistically compared with each other. These chemometric calibrations were successfully applied to the marketed tablets without any separation procedure. Mean recoveries (percent) and relative standard deviation of ILS, CLS, PCR, PLS methods were found to be 98.77/1.76, 100.59/1.53, 97.91/1.50, 97.53/1.73 for DOX; 99.79/1.22, 100.22/0.58, 100.31/1.68 and 99.33/1.10 for PYR; 99.79/1.37, 100.57/1.56 and 98.38/0.96 for FA respectively. All of the four chemometric methods in this study can be satisfactorily used for the quantitative analysis of multi-component dosage form.